Composite

Part:BBa_K4458004

Designed by: Maxwell Swann   Group: iGEM22_East_Coast_BioCrew   (2022-09-10)


pcbA1 assembly

pcbA1 is a reductive dehalogenase found in Dehalococcoides mccartyi, a organohalide respiring bacteria with a natural ability to dechlorinate polychlorinated biphenyls. pcbA1 is one of the few genes conclusively linked to the dechlorination of polychlorinated biphenyls. Found from the paper Growth of Dehalococcoides spp. and increased abundance of reductive dehalogenase genes in anaerobic PCB-contaminated sediment microcosms (Ewald JM, Humes SV, Martinez A, Schnoor JL, Mattes TE. Growth of Dehalococcoides spp. and increased abundance of reductive dehalogenase genes in anaerobic PCB-contaminated sediment microcosms. Environ Sci Pollut Res Int. 2020 Mar;27(9):8846-8858. doi: 10.1007/s11356-019-05571-7. Epub 2019 Jun 17. PMID: 31209752; PMCID: PMC6918016.)

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 13
    Illegal XbaI site found at 234
    Illegal PstI site found at 1
    Illegal PstI site found at 131
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1
    Illegal PstI site found at 131
    Illegal NotI site found at 19
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 700
    Illegal XhoI site found at 805
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 13
    Illegal XbaI site found at 234
    Illegal PstI site found at 1
    Illegal PstI site found at 131
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 13
    Illegal XbaI site found at 234
    Illegal PstI site found at 1
    Illegal PstI site found at 131
    Illegal NgoMIV site found at 1342
    Illegal AgeI site found at 1352
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 343
    Illegal BsaI.rc site found at 415


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Categories
Parameters
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